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pentr/d-topo vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pentr/d-topo vector
    Pentr/D Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+d+topo+vector/pmc11760145-45-7-9
    Average 90 stars, based on 1 article reviews
    pentr/d-topo vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: MicroRNA Tae-miR1130p targets wheat ferroportin1 ( TaFPN1 ) in the absence of iron-responsive element/iron-regulatory protein1 module
    Article Snippet: Firstly, an entry clone of TaFPN1 was generated by sub-cloning the gene in the pENTRTM/D-TOPOTM vector followed by recombination of the TaFPN1 gene fragment in the destination vector;pGWB520 using LR clonase II enzyme mix (Thermofisher Scientific).

    Amplification:

    Article Title: Rice OBF binding protein 4 (OsOBP4) participates in flowering and regulates salt stress tolerance in Arabidopsis
    Article Snippet: .. The full-length CDS of OsOBP4 without the stop codon was amplified from the ‘Pokkali’ rice DNA, and the PCR product was cloned into the pENTR/D-topo vector (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: CUP-SHAPED COTYLEDON2 activates MIR319C transcription and promotes cell proliferation in Arabidopsis leaf primordia
    Article Snippet: .. The full length (2.7 kb) and the non-overlapping promoter fragments, i.e., -2694-1866 ( p distal ), -1866-1107 ( p middle ), and -1107+35 ( p proximal ) of MIR319C were amplified from pMIR319C-P4P1R entry clone using Taq DNA polymerase and cloned into pENTR/D-TOPO vector (K240020, Invitrogen, USA) according to the manufacturer’s instructions. ..

    Polymerase Chain Reaction:

    Article Title: Rice OBF binding protein 4 (OsOBP4) participates in flowering and regulates salt stress tolerance in Arabidopsis
    Article Snippet: .. The full-length CDS of OsOBP4 without the stop codon was amplified from the ‘Pokkali’ rice DNA, and the PCR product was cloned into the pENTR/D-topo vector (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Clinical and Functional Studies of MTOR Variants in Smith-Kingsmore Syndrome Reveal Deficits of Circadian Rhythm and Sleep-Wake Behavior.
    Article Snippet: The wild-type human MTOR cDNA template was from pcDNA3-CMV-Flag-MTOR (Addgene #26603), in which a Flag tag was inserted at the N-terminus and the gene was under the control of the CMV promoter. .. The MTOR PCR fragment was cloned into the pENTR/D-TOPO vector (Invitrogen). .. All MTOR mutations were generated in the parental pENTR-D-MTOR vector via sitedirected mutagenesis using reverse PCR followed by treatment with the KLD enzyme mix (NEB M0554S).

    Article Title: Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization
    Article Snippet: .. The PCR product, which included the entire ORF, was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific, Catalogue #K240020). ..

    Clone Assay:

    Article Title: Rice OBF binding protein 4 (OsOBP4) participates in flowering and regulates salt stress tolerance in Arabidopsis
    Article Snippet: .. The full-length CDS of OsOBP4 without the stop codon was amplified from the ‘Pokkali’ rice DNA, and the PCR product was cloned into the pENTR/D-topo vector (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Clinical and Functional Studies of MTOR Variants in Smith-Kingsmore Syndrome Reveal Deficits of Circadian Rhythm and Sleep-Wake Behavior.
    Article Snippet: The wild-type human MTOR cDNA template was from pcDNA3-CMV-Flag-MTOR (Addgene #26603), in which a Flag tag was inserted at the N-terminus and the gene was under the control of the CMV promoter. .. The MTOR PCR fragment was cloned into the pENTR/D-TOPO vector (Invitrogen). .. All MTOR mutations were generated in the parental pENTR-D-MTOR vector via sitedirected mutagenesis using reverse PCR followed by treatment with the KLD enzyme mix (NEB M0554S).

    Article Title: CUP-SHAPED COTYLEDON2 activates MIR319C transcription and promotes cell proliferation in Arabidopsis leaf primordia
    Article Snippet: .. The full length (2.7 kb) and the non-overlapping promoter fragments, i.e., -2694-1866 ( p distal ), -1866-1107 ( p middle ), and -1107+35 ( p proximal ) of MIR319C were amplified from pMIR319C-P4P1R entry clone using Taq DNA polymerase and cloned into pENTR/D-TOPO vector (K240020, Invitrogen, USA) according to the manufacturer’s instructions. ..

    Article Title: Genotype-environment association across precipitation regimens reveals the mechanism of plant adaptation to rainy environments
    Article Snippet: .. This was then cloned into the pENTR/D-TOPO vector, and the resulting entry vector was recombined into the plasmid p2GW7L using Gateway LR Clonase II (Thermo-Fisher Scientific). ..

    Article Title: Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization
    Article Snippet: .. The PCR product, which included the entire ORF, was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific, Catalogue #K240020). ..

    Article Title: Tissue flows are tuned by actomyosin-dependent mechanics in developing embryos
    Article Snippet: .. The fragment was cloned into the pENTR/D-Topo vector (Life Technologies) and recombined into UASp-attB destination vector (gift from F. Wirtz-Peitz) using the Gateway cloning system (Life Technologies) for subsequent expression using the Gal4/UAS system [ 68 ]. .. The plasmid was inserted into the attP40 site on chromosome II to generate transgenic flies (Bestgene).

    Plasmid Preparation:

    Article Title: Clinical and Functional Studies of MTOR Variants in Smith-Kingsmore Syndrome Reveal Deficits of Circadian Rhythm and Sleep-Wake Behavior.
    Article Snippet: The wild-type human MTOR cDNA template was from pcDNA3-CMV-Flag-MTOR (Addgene #26603), in which a Flag tag was inserted at the N-terminus and the gene was under the control of the CMV promoter. .. The MTOR PCR fragment was cloned into the pENTR/D-TOPO vector (Invitrogen). .. All MTOR mutations were generated in the parental pENTR-D-MTOR vector via sitedirected mutagenesis using reverse PCR followed by treatment with the KLD enzyme mix (NEB M0554S).

    Article Title: Genotype-environment association across precipitation regimens reveals the mechanism of plant adaptation to rainy environments
    Article Snippet: .. This was then cloned into the pENTR/D-TOPO vector, and the resulting entry vector was recombined into the plasmid p2GW7L using Gateway LR Clonase II (Thermo-Fisher Scientific). ..

    Article Title: Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization
    Article Snippet: .. The PCR product, which included the entire ORF, was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific, Catalogue #K240020). ..

    Article Title: Recombinant LAC polynucleotides and uses thereof to increase production of C-lignin in plants
    Article Snippet: .. Table 4, ligated into the pENTR/D-TOPO vector (Invitrogen), and subcloned into pDEST17 fused with a 6× histidine tag at the N-terminus via Gateway LR recombination reaction (Invitrogen). .. The resulting vector (pDEST17-ChLAC8) was transformed into E. coli Rosetta (DE3) (Novagen) for protein expression.

    Article Title: Tissue flows are tuned by actomyosin-dependent mechanics in developing embryos
    Article Snippet: .. The fragment was cloned into the pENTR/D-Topo vector (Life Technologies) and recombined into UASp-attB destination vector (gift from F. Wirtz-Peitz) using the Gateway cloning system (Life Technologies) for subsequent expression using the Gal4/UAS system [ 68 ]. .. The plasmid was inserted into the attP40 site on chromosome II to generate transgenic flies (Bestgene).

    Cloning:

    Article Title: Tissue flows are tuned by actomyosin-dependent mechanics in developing embryos
    Article Snippet: .. The fragment was cloned into the pENTR/D-Topo vector (Life Technologies) and recombined into UASp-attB destination vector (gift from F. Wirtz-Peitz) using the Gateway cloning system (Life Technologies) for subsequent expression using the Gal4/UAS system [ 68 ]. .. The plasmid was inserted into the attP40 site on chromosome II to generate transgenic flies (Bestgene).

    Expressing:

    Article Title: Tissue flows are tuned by actomyosin-dependent mechanics in developing embryos
    Article Snippet: .. The fragment was cloned into the pENTR/D-Topo vector (Life Technologies) and recombined into UASp-attB destination vector (gift from F. Wirtz-Peitz) using the Gateway cloning system (Life Technologies) for subsequent expression using the Gal4/UAS system [ 68 ]. .. The plasmid was inserted into the attP40 site on chromosome II to generate transgenic flies (Bestgene).



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